Difference between revisions of "Endy:FACS protocols"

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==Media==
 
==Media==
As desribed in the standard protocol we often use [[M9_media/supplemented|Supplemented M9 media]] although we are now switching to using [[Neidhardt_EZ_Rich_Defined|EZ media]] where possible.
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As described in the standard protocol we often use [[M9_medium/supplemented|Supplemented M9 media]] although we are now switching to using [[Neidhardt_EZ_Rich_Defined|EZ media]] where possible.
  
 
==Bead calibration==
 
==Bead calibration==
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==Data analysis==
 
==Data analysis==
We do all our analysis using [http://www.flowjo.com/ FloJo] and [http://mathworks.com Matlab].
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We do all our analysis using [http://www.flowjo.com/ FlowJo] and [http://mathworks.com Matlab].
  
 
''Information aggregated by [[Barry Canton]].''
 
''Information aggregated by [[Barry Canton]].''
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[[Category:Protocol]] [[Category:Flow cytometry]]

Latest revision as of 07:51, 5 September 2007

Protocol

Here is a basic standard protocol that we try and use in the Endy and Knight labs. Exact conditions may depend on the strain, induction conditions etc.

Equipment

When we are doing flow experiments, we normally use a FACScan cytometer, described here. Some lab members have experience using the MoFlo machines also. We also have an NPE flow cytometer that we use in our lab for some experiments.

Media

As described in the standard protocol we often use Supplemented M9 media although we are now switching to using EZ media where possible.

Bead calibration

We calibrate results from day to day using this bead protocol.

Data analysis

We do all our analysis using FlowJo and Matlab.

Information aggregated by Barry Canton.