Elizabeth Polidan Week5

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Elizabeth Polidan

BIOL 398.03 / MATH 388

  • Loyola Marymount University
  • Los Angeles, CA, USA

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Saccharomyces Genome Database - SGD

  • Which of these genes has a homolog (similar gene related by descent) in humans? What disease does a deficiency of this gene cause in humans?
    • GDH1, GDH2, and GDH3 have homologs in humans.
      • Deficiencies in the human homologs of glutamate dehydrogenase have been linked to hyperinsulinism-hyperammonemia syndrome and neurological disorders.
  • How is the expression of each of these genes regulated?
    • GDH1 – expression regulated by Carbon and Nitrogen sources; expression high for both ethanol and glucose
    • GDH3 – expression regulated by Carbon and Nitrogen sources; induced by ethanol and repressed by glucose
    • GDH2 – expression regulated by ammonia concentration
    • GLN1 – expression regulated by nitrogen source and by amino acid limitation
    • GLT1 – expression regulated by nitrogen source
  • Using the compound search tool of SGD, search on "L-glutamate". How many pathways does it participate in?
    • L-glutamate participation in pathway reactions
      • As a reactant: 26
      • As a product: 30
  • Find the SGD representation of the pathway we are working on in class and attach a screenshot and hyperlink to your journal page. Choose the one that shows all of the reactions we talked about in class and make sure you can relate it to your notes, matching the genes, enzyme names, and reactants/products.

SGD Pathway SGD.jpg

  • What parameters for these reactions can you find using this database? HINT: the literature portion of the individual gene pages may be helpful.
    • Answer

Kyoto Encyclopedia of Genes and Genomes - KEGG KEGG Pathway KEGG.jpg

  • KEGG also uses a system where a "master" summary pathway compiled from many different organisms is then highlighted with the organism-specific enzymes/genes. How many genes in this pathway exist in yeast?
    • Answer
  • Click on each of the five enzymes of our pathway and read the individual enzyme pages. Is there any new information here that was not represented by SGD?
    • Answer

Reactome Reactome REACTOME.jpg