Difference between revisions of "Electroporation"

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==Specific Protocols==
 
==Specific Protocols==
 
*[[Knight:Electroporation]]
 
*[[Knight:Electroporation]]
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==Notes==
 
==Notes==
  
If you are in a hurry and your selection marker is ampicillin, you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect.
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*If you are in a hurry and your selection marker is ampicillin, you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect.
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*The [[Endy Lab]] is trying to use a standard positive control to better compare (and hopefully improve) the transformation efficiencies in the lab, you can check it out [[Endy:Standard transformation positive control|here]].
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[[Category:E.Coli Protocol]]
 
[[Category:E.Coli Protocol]]

Revision as of 13:08, 20 July 2006

Specific Protocols


Notes

  • If you are in a hurry and your selection marker is ampicillin, you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect.
  • The Endy Lab is trying to use a standard positive control to better compare (and hopefully improve) the transformation efficiencies in the lab, you can check it out here.