Difference between revisions of "Electroporation"

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(Specific Protocols)
 
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{{back to protocols}}
 
==Specific Protocols==
 
==Specific Protocols==
 
*[[Knight:Electroporation]]
 
*[[Knight:Electroporation]]
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*[[Endy:High-efficiency electroporation]]
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*[[Richard Lab:Electroporation of E. coli]]
  
 
==Notes==
 
==Notes==
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*If you are in a hurry and your selection marker is ampicillin and you're sure to have enough plasmid (e.g. not after difficult clonings), you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect. This is called [http://www.neb.com/nebecomm/products/protocol120.asp 5-minute transformation] by New England Biolabs and if you follow the link you'll get a nice complete protocol for it (10% efficiency compared to full protocol)
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*The [[Endy Lab]] is trying to use a standard positive control to better compare (and hopefully improve) the transformation efficiencies in the lab, you can check it out [[Endy:Standard transformation positive control|here]].
  
If you are in a hurry and your selection marker is ampicillin, you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect.
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[[Category:Protocol]]
[[Category:E.Coli Protocol]]
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[[Category:In vivo]]
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[[Category:Escherichia coli]]

Latest revision as of 10:40, 23 June 2009

back to protocols

Specific Protocols

Notes

  • If you are in a hurry and your selection marker is ampicillin and you're sure to have enough plasmid (e.g. not after difficult clonings), you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect. This is called 5-minute transformation by New England Biolabs and if you follow the link you'll get a nice complete protocol for it (10% efficiency compared to full protocol)
  • The Endy Lab is trying to use a standard positive control to better compare (and hopefully improve) the transformation efficiencies in the lab, you can check it out here.