Here are some protocols we use in the lab.
- Extracting soluble and insoluble protein fractions
- Thoughts on lysis of yeast cells
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model