Difference between revisions of "Drummond:Protocols"

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(Local Protocols)
(rather rapid alternative)
 
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{{Drummond_Top}}
 
{{Drummond_Top}}
 
<div style="width: 750px">
 
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__NOTOC__
 
==Local Protocols==
 
==Local Protocols==
 
Here are some protocols we use in the lab.
 
Here are some protocols we use in the lab.
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*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]]
 
*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]]
 
*[[Drummond:Transformation|High-efficiency yeast transformation]]
 
*[[Drummond:Transformation|High-efficiency yeast transformation]]
*[[Drummond:DNA Prep|Yeast genomic DNA prep]]
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*[[Drummond:DNA Prep|Yeast genomic DNA prep]] (consider the [http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html rather rapid yeast genomic DNA prep] instead)
 
*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]]
 
*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]]
 
*[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]]
 
*[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]]
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==Remote Protocols==
 
==Remote Protocols==
 
*[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)]
 
*[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)]
*[http://www.fhcrc.org/science/labs/gottschling/yeast/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)]
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*[http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)]
 
*[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)]
 
*[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)]
  

Latest revision as of 07:47, 13 October 2012

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the drummond lab

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Local Protocols

Here are some protocols we use in the lab.

Remote Protocols

Data Analysis Protocols

Notes