Drummond:Protocols: Difference between revisions
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==Local Protocols== | ==Local Protocols== | ||
Here are some protocols we use in the lab. | Here are some protocols we use in the lab. | ||
*[[Drummond: | *[[Drummond:ChlorMethExtraction|Chloroform/methanol precipitation of proteins]] | ||
*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | *[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | ||
*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | *[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | ||
*[[Drummond:Fluorescence|Cell preparation and protocol to view RFP/TFP fluorescence]] | *[[Drummond:Fluorescence|Cell preparation and protocol to view RFP/TFP fluorescence]] | ||
*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]] | *[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]] | ||
*[[Drummond:Viability|Yeast OD and cell viability model]] | |||
*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]] | |||
*[[Drummond:Transformation|High-efficiency yeast transformation]] | |||
*[[Drummond:DNA Prep|Yeast genomic DNA prep]] (consider the [http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html rather rapid yeast genomic DNA prep] instead) | |||
*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]] | |||
*[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]] | |||
*[[Drummond:Anti-Ubiquitin Beads|Preparing anti-ubiquitin beads]] | |||
*[[Drummond:Periplasmic Prep|Periplasmic protein prep from <i>E. coli</i>]] | |||
<!-- *[[Drummond:Solubility|Extracting soluble and insoluble protein fractions]] --> | |||
==Remote Protocols== | ==Remote Protocols== | ||
[http:// | *[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)] | ||
[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)] | *[http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)] | ||
*[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)] | |||
==Data Analysis Protocols== | |||
*[[Drummond:Akashi's Test|Akashi's test for translational accuracy selection]] | |||
==Notes== | |||
*[[Drummond:PopGen|The relationship between the selection coefficient and relative growth rates: under construction]] | |||
*[[Drummond:Lysis|Thoughts on lysis of yeast cells]] | |||
</div> | </div> | ||
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Latest revision as of 07:47, 13 October 2012
Local Protocols
Here are some protocols we use in the lab.
- Chloroform/methanol precipitation of proteins
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- Standard conditions for flow cytometer growth rate assay
- High-efficiency yeast transformation
- Yeast genomic DNA prep (consider the rather rapid yeast genomic DNA prep instead)
- Isolating total, soluble and insoluble protein fractions
- Making chemically competent E. coli cells
- Preparing anti-ubiquitin beads
- Periplasmic protein prep from E. coli
Remote Protocols
- Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)
- Rather rapid yeast genomic DNA prep (Gottschling Lab)
- SDS-PAGE (Szostak Lab)
