(1% SDS, 8M Urea, 10mM MOPS, pH 6.8, 10mM EDTA)
To make 100ml:
* 1.0ml 1M MOPS, pH6.8 stock solution
* 1.0g SDS, or 10ml 10% SDS stock solution
* 48.05g Urea
* 2.0ml 0.5M EDTA stock solution
This is used for lysing yeast at pH6.8. For pH8 lyses, use the variation of SUME with Tris buffer known as SUTE.
from Yeast Protocols
3-(N-tetradecyl-N,N-dimethyl-ammonio)-propane-1-sulfonate (SB14) , available from Sigma as SB3-14.
- Zanna L and Haeuw JF. Separation and quantitative analysis of alkyl sulfobetaine-type detergents by high-performance liquid chromatography and light-scattering detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Feb 1;846(1-2):368-73. DOI:10.1016/j.jchromb.2006.08.036 |