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SUME Buffer (1% SDS, 8M Urea, 10mM MOPS, pH 6.8, 10mM EDTA) To make 100ml:

   * 1.0ml 1M MOPS, pH6.8 stock solution
   * 1.0g SDS, or 10ml 10% SDS stock solution
   * 48.05g Urea
   * 2.0ml 0.5M EDTA stock solution

This is used for lysing yeast at pH6.8. For pH8 lyses, use the variation of SUME with Tris buffer known as SUTE.

from Yeast Protocols

Chemical lysis

Myristyl sulfobetaine 3-(N-tetradecyl-N,N-dimethyl-ammonio)-propane-1-sulfonate (SB14) [1], available from Sigma as SB3-14.


  1. Zanna L and Haeuw JF. Separation and quantitative analysis of alkyl sulfobetaine-type detergents by high-performance liquid chromatography and light-scattering detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Feb 1;846(1-2):368-73. DOI:10.1016/j.jchromb.2006.08.036 | PubMed ID:16978932 | HubMed [ZannaJChr07]