Drummond:Lysis

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SUME Buffer (1% SDS, 8M Urea, 10mM MOPS, pH 6.8, 10mM EDTA) To make 100ml:

   * 1.0ml 1M MOPS, pH6.8 stock solution
   * 1.0g SDS, or 10ml 10% SDS stock solution
   * 48.05g Urea
   * 2.0ml 0.5M EDTA stock solution

This is used for lysing yeast at pH6.8. For pH8 lyses, use the variation of SUME with Tris buffer known as SUTE.

from Yeast Protocols