Difference between revisions of "DpnI"
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Revision as of 09:05, 27 May 2007
- To digest methylated GATC sites. Useful for removing cell-derived plasmid template from PCR samples.
- Can be ordered from NEB.
- Typically stored at -20°C.
- Use in NEBuffer 4. However, can be added directly to PCR sample.
- Incubate at 37°C.
- Heat inactivate by incubating at 80°C for 20 minutes.
- None known.