Difference between revisions of "DpnI"

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==Contact==
 
==Contact==
 
*[[Reshma Shetty]]
 
*[[Reshma Shetty]]
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[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]

Revision as of 09:05, 27 May 2007

Purpose

  • To digest methylated GATC sites. Useful for removing cell-derived plasmid template from PCR samples.

Procurement

  • Can be ordered from NEB.
  • Typically stored at -20°C.

Use

  • Use in NEBuffer 4. However, can be added directly to PCR sample.
  • Incubate at 37°C.
  • Heat inactivate by incubating at 80°C for 20 minutes.

Relevant protocols

Safety

  • None known.

References

Contact