Difference between revisions of "Disrupting the biofilm"

From OpenWetWare
Jump to: navigation, search
Line 2: Line 2:
== Biofilm disruption solution ==
== Biofilm disruption solution ==
* 1% SDS [http://en.wikipedia.org/wiki/Sodium_dodecyl_sulfate Sodium dodecyl sulfate]
* 1% SDS [http://en.wikipedia.org/wiki/Sodium_dodecyl_sulfate Sodium dodecyl sulfate] (denaturates protein)
* 5 mM [http://en.wikipedia.org/wiki/Edta EDTA]
* 5 mM [http://en.wikipedia.org/wiki/Edta EDTA]
* 100 mM NaCl
* 100 mM NaCl

Latest revision as of 13:29, 15 October 2007

The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain composition in each biofilm in a high. For this, we need to disrupt the biofilm back into suspended cells so that we can quantify their relative amounts through fluorescence.

Biofilm disruption solution


  • Grow biofilm in 96-well plate.
  • Extract liquid (measure OD if necessary) and wash twice.
  • Fill with 150 μm disruption solution.
  • Shake for 30 mins.
  • Measure OD.
  • Measure fluorescences.