Difference between revisions of "Disrupting the biofilm"

From OpenWetWare
(New page: The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain compo...)
 
Line 2: Line 2:
  
 
== Biofilm disruption solution ==
 
== Biofilm disruption solution ==
* 1% SDS
+
* 1% SDS [http://en.wikipedia.org/wiki/Sodium_dodecyl_sulfate Sodium dodecyl sulfate]
* 5 mM EDTA
+
* 5 mM [http://en.wikipedia.org/wiki/Edta EDTA]
 
* 100 mM NaCl
 
* 100 mM NaCl
* 50 mM Tris  
+
* 50 mM [http://en.wikipedia.org/wiki/Tris Tris]
 
* pH 7.4
 
* pH 7.4

Revision as of 12:46, 12 October 2007

The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain composition in each biofilm in a high. For this, we need to disrupt the biofilm back into suspended cells so that we can quantify their relative amounts through fluorescence.

Biofilm disruption solution