Difference between revisions of "Dionne"

From OpenWetWare
Jump to: navigation, search
Line 27: Line 27:
 
<nonwikionly>
 
<nonwikionly>
 
<a href="http://www.openwetware.org"><img src="http://openwetware.org/images/9/96/02_JoinOpenWetWare.png" border=0/></a> </nonwikionly>
 
<a href="http://www.openwetware.org"><img src="http://openwetware.org/images/9/96/02_JoinOpenWetWare.png" border=0/></a> </nonwikionly>
 
</div>
 

Revision as of 08:21, 8 May 2007

Imperial-banner.png

About Us       Protocols &c.       Lab Members       Publications       Contact       Links


Welcome to the Dionne lab!

We are interested in (1) the effects of host genetics on the biology of infection; and (2) the physiological control of metabolic balance.

Host genetics and the biology of infection

Different individuals show different levels of resistance to infections and develop different pathologies in response to infections. We are interested in why this is the case. We use the fruitfly Drosophila melanogaster as a model host to study these questions; this allows us to screen for genes that affect the progress of infection in a rapid and unbiased fashion.

All of our experiments originate from a simple genetic screen. Mutant flies are infected with Mycobacterium marinum, a bacterium closely-related to the causative agent of tuberculosis, or with Mycobacterium smegmatis, a related non-pathogen. We select lines of flies that die more quickly or more slowly than wild-type controls and identify the mutation that gives rise to this phenotype. We then try to understand what this phenotype tells us about the function of the mutated gene.

So far, our work on this system has focused on the mechanisms of pathogenesis. We have found that this infection causes progressive loss of metabolic stores, similar to the wasting seen in people with tuberculosis. We have shown that, in the fly, this wasting effect is caused partly by systemic failures in anabolic signals via the insulin effector kinase Akt. We are now working to try to understand how infection causes this defect in anabolic signalling. We also have mutants that affect other aspects of disease; we are working with these mutants to understand other aspects of disease pathogenesis as well as how the fly immune system fights Mycobacterial infections.

Physiological control of metabolic balance

As mentioned above, we've found that infection with M marinum causes serious metabolic defects in Drosophila. At least some of these effects are due to changes in signalling pathways whose roles in metabolic control are largely unexplored or completely unknown. This has led us to examine the roles of these pathways in metabolic control in healthy animals so that we can then understand the effects of infection-induced perturbation of these pathways.

This work is preliminary but is very exciting - we hope to be able to say more soon!

<wikionly>

Recent updates to the lab wiki

22 May 2018

     01:58  Lee:Lab Members‎ (diff | hist) . . (+84). . Wooin Lee (talk | contribs) (Research rotation students)

21 May 2018

     23:05  Lee:Lab Members‎ (diff | hist) . . (+11). . Wooin Lee (talk | contribs) (Alumni)

     12:15 

Endy Lab‎‎ (2 changes | history) . . (+302). . [Reicker1‎ (2×)]

     

12:15

(cur | prev) . . (+93). . Reicker1 (talk | contribs) (News)

     

12:11

(cur | prev) . . (+209). . Reicker1 (talk | contribs) (News)

     11:57 (User creation log) . . User account Cuervito Naranja (talk | contribs) was created by Yar (talk | contribs) and password was sent by email ‎
     10:54  Chenlab‎ (diff | hist) . . (0). . Kaifu Chen (talk | contribs)

     10:44 

Chenlab:Publications‎‎ (5 changes | history) . . (+161). . [Kaifu Chen‎ (5×)]

     

10:44

(cur | prev) . . (+92). . Kaifu Chen (talk | contribs)

     

10:42

(cur | prev) . . (+5). . Kaifu Chen (talk | contribs)

     

10:41

(cur | prev) . . (+12). . Kaifu Chen (talk | contribs)

     

10:40

(cur | prev) . . (+17). . Kaifu Chen (talk | contribs)

     

10:40

(cur | prev) . . (+35). . Kaifu Chen (talk | contribs)

     09:34  CAMRI:SeminarSeries‎ (diff | hist) . . (+110). . Lexi E. Crommett (talk | contribs)
     02:56  Lee:Publications‎ (diff | hist) . . (+12). . Wooin Lee (talk | contribs) (2018)

     00:18 

Peyton:Internal‎‎ (4 changes | history) . . (+132). . [Hyunakim16‎ (4×)]

     

00:18

(cur | prev) . . (0). . Hyunakim16 (talk | contribs) (Protocols)

     

00:17

(cur | prev) . . (+9). . Hyunakim16 (talk | contribs)

     

00:14

(cur | prev) . . (-1). . Hyunakim16 (talk | contribs)

     

00:11

(cur | prev) . . (+124). . Hyunakim16 (talk | contribs) (Protocols)

     00:12 (Upload log) . . Hyunakim16 (talk | contribs) uploaded File:PEG-Maleimide mechanical testing (CAT) HK 20180507 v3.pdf

20 May 2018

     23:30  Qin‎ (diff | hist) . . (+98). . Yaning (talk | contribs) (News)
     23:20  Sangdunchoi:Alumini‎ (diff | hist) . . (+323). . Mahesh Chandra Patra (talk | contribs) (Alumni)

     23:19 

Peyton:Internal‎‎ (2 changes | history) . . (+94). . [Hyunakim16‎ (2×)]

     

23:19

(cur | prev) . . (-1). . Hyunakim16 (talk | contribs)

     

23:13

(cur | prev) . . (+95). . Hyunakim16 (talk | contribs)

     23:17 

Sangdunchoi:Members‎‎ (5 changes | history) . . (-507). . [Mahesh Chandra Patra‎ (5×)]

     

23:17

(cur | prev) . . (+7). . Mahesh Chandra Patra (talk | contribs) (Postdoctoral Researcher)

     

23:16

(cur | prev) . . (+7). . Mahesh Chandra Patra (talk | contribs) (Postdoctoral Researcher)

     

23:16

(cur | prev) . . (-50). . Mahesh Chandra Patra (talk | contribs) (Postdoctoral Researcher)

     

23:14

(cur | prev) . . (-902). . Mahesh Chandra Patra (talk | contribs) (PhD Students)

     

22:22

(cur | prev) . . (+431). . Mahesh Chandra Patra (talk | contribs) (Postdoctoral Researcher)

     23:15 (Upload log) . . Hyunakim16 (talk | contribs) uploaded File:RNA extraction from gels HK 20170918 v8.pdf
     23:09  Sangdun Choi:Professor‎ (diff | hist) . . (-134). . Mahesh Chandra Patra (talk | contribs)
     22:19  Sangdun Choi:Researcher papers‎ (diff | hist) . . (-135). . Mahesh Chandra Patra (talk | contribs)
     14:22  Hoatlin:BMB seminar series 2017-2018‎ (diff | hist) . . (+72). . Maureen E. Hoatlin (talk | contribs) (Seminar Series Planning 2018-2019)
     08:06  Lee:JC‎ (diff | hist) . . (-42). . Wooin Lee (talk | contribs) (2018)

19 May 2018

     11:47 (User creation log) . . User account Yaning (talk | contribs) was created by Yar (talk | contribs) and password was sent by email ‎

18 May 2018

     20:02 

User:David S LeBauer‎‎ (3 changes | history) . . (-1,044). . [David S LeBauer‎ (3×)]

     

20:02

(cur | prev) . . (-13). . David S LeBauer (talk | contribs) (I've moved my home page: https://davidlebauer.com)

     

20:02

(cur | prev) . . (-22). . David S LeBauer (talk | contribs)

     

20:02

(cur | prev) . . (-1,009). . David S LeBauer (talk | contribs) (Replaced content with "{{Template:DLeBauer}} ==I've moved my home page: https://davidlebauer.com ==")

     15:00  Endy:Stanford Lab Meetings‎ (diff | hist) . . (+37). . Reicker1 (talk | contribs) (Endy Lab Meetings Spring 2018)

     14:52 

Beauchamp:Orientation‎‎ (3 changes | history) . . (-51). . [Michael S Beauchamp‎ (3×)]

     

14:52

(cur | prev) . . (-20). . Michael S Beauchamp (talk | contribs)

     

14:52

(cur | prev) . . (-1). . Michael S Beauchamp (talk | contribs)

     

14:52

(cur | prev) . . (-30). . Michael S Beauchamp (talk | contribs)

     13:37 (User creation log) . . User account Netnapa (talk | contribs) was created by Yar (talk | contribs) and password was sent by email ‎

     12:15 

Beauchamp:RestingState‎‎ (8 changes | history) . . (+3,923). . [Meghanr‎ (8×)]

     

12:15

(cur | prev) . . (+1,207). . Meghanr (talk | contribs) (Seed-based approach)

     

11:47

(cur | prev) . . (+1,229). . Meghanr (talk | contribs) (Example afni_proc.py script - some reasonable choices for noise regression)

     

11:36

(cur | prev) . . (+67). . Meghanr (talk | contribs) (Example afni_proc.py script - some reasonable choices for noise regression)

     

11:36

(cur | prev) . . (+139). . Meghanr (talk | contribs) (Example afni_proc.py script - some reasonable choices for noise regression)

     

11:34

(cur | prev) . . (+262). . Meghanr (talk | contribs) (Overall approach)

     

11:04

(cur | prev) . . (-5). . Meghanr (talk | contribs) (Seed-based approach)

     

09:52

(cur | prev) . . (+43). . Meghanr (talk | contribs) (Seed-based approach)

     

09:51

(cur | prev) . . (+981). . Meghanr (talk | contribs) (Example afni_proc.py script - some reasonable choices for noise regression)

     11:27  Beauchamp:Lab Notebook‎ (diff | hist) . . (-6). . Meghanr (talk | contribs)
</wikionly>

<nonwikionly> <a href="http://www.openwetware.org"><img src="http://openwetware.org/images/9/96/02_JoinOpenWetWare.png" border=0/></a> </nonwikionly>