Difference between revisions of "Dilkes:Research"

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(New page: =Notes on Construction= Yup, Beth Toener would be proud. This page is currently under construction. Check back for protocols and updates. ==Submission Procedures and Internal Protocols h...)
 
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=Notes on Construction=
 
Yup, Beth Toener would be proud.
 
This page is currently under construction. Check back for protocols and updates.
 
  
==Submission Procedures and Internal Protocols here at the UC Davis!==
 
# To get [[Davis Sequencing Center Submission| sequence from a few samples]] try [Davis Sequencing Center]
 
# For more than 30 samples it is more economical to use the Genomics Facility run by Doug Cook and compatriots.
 
## We do sequencing direct from PCR products via an [[Exo Sap]] treatment prior to submission
 
## [[CGF Submission]] is relatively straightforward but requires that you set up an account with them.
 
# There are off campus options including Robert Tran of the Davis Tilling Facility's favorite at [[Rob Tran's Favorite sequencing]]
 
 
==Comai Lab Primer Procedures==
 
# Using the primer database to find your primer
 
# Ordering primers not in the collection
 
# Adding new entries to the primer database
 
 
==Comai Lab Polyploidy Procedures==
 
# Fluorescent genotyping
 
# Aneuploidy detection
 
# FISH Protocol
 
# Pollen staining and viability measurements
 
# Seed phenotyping
 
# GFP and enhancer trap PCR methods
 
# GFP imaging
 
# Sample labeling for two color CGH on Arabidopsis Operon arrays (lifted from the good people at [http://www.fhcrc.org FHCRC array core])
 

Latest revision as of 05:29, 18 January 2010