Difference between revisions of "Defining the network of ROP GTPase signalling and its regulation in root hair development"

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I am studying the molecular basis of Rho GTPase signalling in root hairs using:
+
I studied the molecular basis of Rho GTPase signalling in root hairs using:
 
*An ''in vitro'' system to test for direct protein-protein interactions between Rho GTPases and candidate interacting proteins.
 
*An ''in vitro'' system to test for direct protein-protein interactions between Rho GTPases and candidate interacting proteins.
 
*Over-expression of candidate proteins to indicate if specific genes play a critical role in root hair development.
 
*Over-expression of candidate proteins to indicate if specific genes play a critical role in root hair development.
 
*Co-expression of Rho GTPases and candidate genes using fluorescent proteins to visualise the sub-cellular localisation of the proteins ''in vivo''.
 
*Co-expression of Rho GTPases and candidate genes using fluorescent proteins to visualise the sub-cellular localisation of the proteins ''in vivo''.

Latest revision as of 00:53, 19 December 2009

Matt Smallman

Contact Info

Matt Smallman School of Biological Sciences, University of Bristol, Woodland Road, Bristol. BS8 1UG UK

matt.smallman@bristol.ac.uk

Grierson Lab page


Rho small GTPases are conserved molecular switches in eukaryotic signal transduction involved in a variety of biological processes such as the establishment of cell polarity and actin remodeling. The GTPase switch is closely controlled through regulatory mechanism involving at least two kinds of negative regulators:


  • Rho Guanine nucleotide Dissociation Inhibitors (GDIs)
  • Rho GTPase activating proteins (GAPs)


ROP GTPase Siganlling


I studied the molecular basis of Rho GTPase signalling in root hairs using:

  • An in vitro system to test for direct protein-protein interactions between Rho GTPases and candidate interacting proteins.
  • Over-expression of candidate proteins to indicate if specific genes play a critical role in root hair development.
  • Co-expression of Rho GTPases and candidate genes using fluorescent proteins to visualise the sub-cellular localisation of the proteins in vivo.