Dahlquist:Yeast Transcription Factors

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Dahlquist Lab Yeast Transcription Factor Resources

This page displays a compiled list of yeast transcription factor resources. Included are data on transcription factor target genes and transcription factor regulatory networks in yeast, links to web servers providing easy-to-use TF searches and other potentially helpful resources.

TF Network Data

  • TF-target interactions through DNA damage response

Measured binding locations of damage-related transcription factors (including Cin5) after exposure to MMS. Validated by checking against binding in knock-out strains for TFs. Paper has a map of the network, and the supplemental material is downloaded but needs to be sifted through.

  • From Workman, Christopher T., et al. A systems approach to mapping DNA damage response pathways. Science 2006. 1054-1059. PubMed
  • TF network though systematic deletions

Performed microarray analysis of knockout strains for many transcription factors and compared expression profiles to determine regulatory relationships. Have a link to supplementary data, need to search through to find information.

  • From Hu, Zhanzhi, et al. Genetic reconstruction of a functional transcriptional regulatory network. Nature Genetics 2007. 39:5. 683-687. PubMed
  • Updated TF network to determine new relationships

Used improved algorithms to detect new regulatory relationships in the transcription factor network. Fig. 3 Shows that the effect on Cin5 was to gain approximately 10 new interactions and lose approximately two as compared to the older model. A figure of the new network was downloaded on the desktop.

  • From MacIsaac, Kenzie D., et al. An improved map of conserved regulatory sites for Saccharomyces cerevisiae. BMC Bioinformatics 2006, 7:113. PubMed

Web Servers

  • MYBS Mining Yeast Binding Sites

The search regulatory associations function allows the user to input a list of genes of interest and search for candidate transcription factors that regulate the list of genes. Allows filtering according to multiple criteria.

  • From Tsai, HK, et al. MYBS: A comprehensive web server for mining transcription factor binding sites in yeast. Nucleic Acids Research 2007. PubMed
  • TFSVM Transcription Factor Support Vector Machine

Allows user to input either a single transcription factor to get a list of regulated target genes or a single gene to get a list of transcription factors that regulate that gene. Can also filter according to p-value.

  • From Holloway, DT, et al. Classifying transcription factor targets and discovering relevant biological features. Biology Direct 2008. 3:22. PubMed
  • YEASTRACT Yeast Search for Transcriptional Regulators and Consensus Tracking

Multiple tools are provided on this server. Those of interest include a search for regulatory relationships either by transcription factor or by target gene. Applies a different algorithm to find new regulatory relationships in previously published data from Lee, Harbison, Tan and Workman.

  • From Monteiro, PT, et al. YEASTRACT-DISCOVERER: new tools to improve analysis of transcriptional regulatory associations in Saccharomyces cerevisiae. Nucleic Acids Research 2008. PubMed

Other Papers of Interest

  • Cold Shock Temporal Network Data

Used new algoithm to construct temporal networks of yeast's response to various stresses, including cold shock. For cold shock data, the paper analyzes data from the Gash, et al. (2000) paper. Supplementary data has cold shock temporal response, but does not show any interactions between transcription factors and targets.

  • From Ernst, J; Vainas, O; Harbison, CT; Simon, I; Bar-Joseph, Z. Reconstructing dynamic regulatory maps. Molecular Systems Biology 2007. PubMed