Difference between revisions of "DNA ladder"

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#100μL 2-log ladder (at 1μg/μL)
 
#100μL 2-log ladder (at 1μg/μL)
 
#200μL loading dye
 
#200μL loading dye
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[[Category:Material]] [[Category:Agarose gel electrophoresis]]

Latest revision as of 07:34, 11 March 2008

Prepared DNA ladder

Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add loading dye and store at 4°C. Load 12 μL per lane for 1 μg of DNA.

To make 1.2 mL prepared ladder, combine:

  1. 900μL deionized water
  2. 100μL 2-log ladder (at 1μg/μL)
  3. 200μL loading dye