Difference between revisions of "DNA blot (Southern)"

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The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
 
The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
  
== Lab-specific DNA blotting protocols ==
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== Lab-specific protocols ==
 
* [[Oneill_Lab:Southern_Blot]]
 
* [[Oneill_Lab:Southern_Blot]]
  
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* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia]
 
* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia]
 
* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia]
 
* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia]
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[[Category:Protocol]]
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[[Category:DNA]]
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[[Category:in vitro]]

Latest revision as of 07:13, 16 April 2009

The DNA blot or Southern blot is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a restriction endonuclease. Fragments are then separated by size using agarose gel electrophoresis. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.

Lab-specific protocols

See also

External links