Illumina GA Data Management
Short read toolbox. Many of our projects use short-read data from the Illumina Genome Analyzer 1 & 2. Brian Knaus in our lab has developed a number of scripts for managing and analyzing short-read files and data.
Illumina GA DNA-Seq
DNA_Seq Prep. Our research group has developed several methods for sequencing small genomes (mitochondria, chloroplasts, BACS) in multiplex using Illumina GA2. This page provides details on DNA-Seq library construction.
Illumina GA RNA-Seq
Illumina GA Hyb-Seq
Hyb_Seq Prep. Like many groups, we've developed customized approaches to enrich rare genomic targets for high-throughput sequencing. Our method for isolating chloroplast genomes by Hyb-Seq is detailed here.
Whole Genome Amplification
WGA Prep. We use phi29-based whole-genome amplification in a variety of different applications. Our standard phi29 WGA method is detailed here.
Random Lab Methods
Random Lab Methods. DNA extraction, RNA extraction, gels, and short cuts... all here
References on the Illumina GA Sequencer
- Illumina support documentation