Difference between revisions of "Cresol Red"

From OpenWetWare
Jump to: navigation, search
(illustration)
m (links)
 
Line 3: Line 3:
 
[[Image:Agarose gel dyes.jpg|right|150px]]
 
[[Image:Agarose gel dyes.jpg|right|150px]]
  
'''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
+
'''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike [[bromophenol blue]] and [[xylene cyanol]], cresol red does not inhibit Taq polymerase in PCRs.
  
 
== Agarose molecular weight marker ==
 
== Agarose molecular weight marker ==

Latest revision as of 06:31, 3 June 2009

chemical structure of cresol red
Agarose gel dyes.jpg

Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.

Agarose molecular weight marker

advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue

  • molecular weight 404 g/mol
  • in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
  • in 3% agarose at >100bp (xylene cyanol 300bp)

pH indicator

  • amber - acidic pH
  • yellow - neutral
  • red - alkaline

External links