Difference between revisions of "Cresol Red"

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[[Image:Cresol red.png|right|150px]]
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[[Image:Cresol red.png|right|150px|thumb|chemical structure of cresol red]]
  
'''Cresol red''' is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
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[[Image:Agarose gel dyes.jpg|right|150px]]
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'''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike [[bromophenol blue]] and [[xylene cyanol]], cresol red does not inhibit Taq polymerase in PCRs.
  
 
== Agarose molecular weight marker ==
 
== Agarose molecular weight marker ==
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* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties]  
 
* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties]  
 
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia]
 
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia]
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[[Category:Material]] [[Category:Chemical]] [[Category:agarose gel electrophoresis]]

Latest revision as of 07:31, 3 June 2009

chemical structure of cresol red
Agarose gel dyes.jpg

Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.

Agarose molecular weight marker

advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue

  • molecular weight 404 g/mol
  • in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
  • in 3% agarose at >100bp (xylene cyanol 300bp)

pH indicator

  • amber - acidic pH
  • yellow - neutral
  • red - alkaline

External links