Cresol Red: Difference between revisions
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== External links == | == External links == | ||
* [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion] | |||
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem] | * [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem] | ||
* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=114480|Aldrich&N5=Product%20No.|BRAND_KEY&F=SPEC Cresol Red sodium salt - Sigma catalogue] | * [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=114480|Aldrich&N5=Product%20No.|BRAND_KEY&F=SPEC Cresol Red sodium salt - Sigma catalogue] | ||
* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties] | * [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties] | ||
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia] | * [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia] | ||
Revision as of 03:51, 14 January 2009
Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
Agarose molecular weight marker
advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue
- molecular weight 404 g/mol
- in 1% agarose at ~125 bp
- in 2% agarose at ~300 bp (between xylene cyanol and bromophenol blue)
pH indicator
- amber - acidic pH
- yellow - neutral
- red - alkaline
