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Protocols for the Noireaux Lab at the University of Minnesota [Physics Department].


  1. Cell Plating
  2. Miniculture
  3. Competent Cells
  4. Transformation
  5. Gel electrophoresis
  6. Miniprep
  7. DNA Purification by PCR Purification Kit
  8. Gel Extraction of DNA
  9. DNA Quantification by Quantifluore
  10. DNA Linker Design
  11. DNA Digestion by Restriction Enzymes
  12. Linker Construction by ssDNA Hybridization
  13. DNA Ligation by T4 Ligase
  14. Polymerase Chain Reaction (PCR)
  15. Whole Plasmid PCR
  16. DNA Sequencing
  17. Cell Free Expression in Batch Mode
  18. Cell Free Expression in Continuous Mode
  19. Cell Free Expression in Phospholipid Vesicles
  20. Plaque Counting


Digital Signature

SC 16:08, 25 June 2012 (EDT)