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Protocols for the Noireaux Lab at the University of Minnesota [Physics Department].


  1. Cell Plating
  2. Miniculture
  3. Competent Cells
  4. Transformation
  5. Gel electrophoresis
  6. Miniprep
  7. DNA Purification by PCR Purification Kit
  8. Gel Extraction of DNA
  9. DNA Quantification by Quantifluore
  10. DNA Digestion by Restriction Enzymes
  11. Linker Construction by ssDNA Hybridization
  12. DNA Ligation by T4 Ligase
  13. Polymerase Chain Reaction (PCR)
  14. Whole Plasmid PCR
  15. Cell Free Expression in Batch Mode
  16. Cell Free Expression in Continuous Mode
  17. Cell Free Expression in Phospholipid Vesicles


or instead, discuss this protocol.

Digital Signature

SC 16:08, 25 June 2012 (EDT)