Corum:DNA Hybridization

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Revision as of 16:55, 19 June 2012 by Sean P Corum (talk | contribs) (New page: '''~~~~''': ==Overview== Hybridization of ssDNA oligonucleotides. ==Materials== * 5 μL 2mM sense oligonucleotide * 5 μL 2mM antisense oligonucleotide ==Procedure== # Combine sense an...)
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SC 20:55, 19 June 2012 (EDT):


Hybridization of ssDNA oligonucleotides.


  • 5 μL 2mM sense oligonucleotide
  • 5 μL 2mM antisense oligonucleotide


  1. Combine sense and antisense oligonucleotides. Vortex and spin.
  2. Seal tube airtight with paraffin and place in weighted holder.
  3. Boil 400 mL water in a 500 mL beaker.
  4. Cool on bench 5 min.
  5. Place weighted holder with sealed oligonucleotides into the beaker.
  6. Incubate on bench overnight or until water is about room temperature.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.


Relevant papers, books, and websites

  1. New England Biolabs


or instead, discuss this protocol.