Difference between revisions of "Christiaen:Methods"

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(animals and embryos)
(animals and embryos)
 
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==animals and embryos==
 
==animals and embryos==
 
[[sea water tank and adult animals]]<br>
 
[[sea water tank and adult animals]]<br>
 +
Most of the methods are described in details in the [http://cshprotocols.cshlp.org/cgi/content/abstract/2009/12/pdb.emo138 CSHL press - Ciona protocols], including :<br>
 
[[dissection and fertilization]]<br>
 
[[dissection and fertilization]]<br>
 
[[dechorionnation]]<br>
 
[[dechorionnation]]<br>

Latest revision as of 07:32, 18 February 2010

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page in construction

this page provides access to the methods referred to as in the beginner's guide to ascidian developmental biology as well as other resources.

animals and embryos

sea water tank and adult animals
Most of the methods are described in details in the CSHL press - Ciona protocols, including :
dissection and fertilization
dechorionnation
Ciona electroporation
microinjection
fluorescence activated cell sorting (FACS)

molecular biology

molecular cloning
microarray analysis

immunohistochemistry and in situ hybridization


microscopy and image analysis