Choano Food Preperation

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Revision as of 15:25, 3 September 2010 by Conor McClune (talk | contribs) (Procedure)
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Procedure

  • Add all 100 or 150uL of an MC1061 competent cell tube to 400mL LB
  • Grow up overnight on 37*C shaker
  • split into two centrifuge bottles and centrifuged at 4200rpm for 14 minutes
  • pour off supernatant
  • wash each pellet with 10mL artificial sea water (ASW)
  • pour off ASW
  • add 10mL ASW to each bottle and resuspend pellets
  • centrifuge at 4200rpm for 14 minutes
  • pour off supernatant
  • re-suspend pellets
  • combine the contents of the two bottles
  • create 2uL aliquots