Difference between revisions of "Chemical transformation buffer comparison"

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|'''Buffer'''
 
|'''Buffer'''
 
|'''KCl'''
 
|'''KCl'''
|'''KCH<sub>3</sub>COO'''
+
|'''KOAc'''
 
|'''CaCl<sub>2</sub>'''
 
|'''CaCl<sub>2</sub>'''
 
|'''MnCl<sub>2</sub>'''
 
|'''MnCl<sub>2</sub>'''
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|'''Glycerol'''
 
|'''Glycerol'''
 
|'''DMSO'''
 
|'''DMSO'''
|'''Sucrose'''
 
 
|'''Other'''
 
|'''Other'''
 
|'''pH'''
 
|'''pH'''
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|
 
|
 
|10%
 
|10%
|
 
 
|
 
|
 
|
 
|
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|
 
|
 
|15%
 
|15%
|
 
 
|
 
|
 
|
 
|
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|
 
|
 
|15%
 
|15%
|
 
 
|
 
|
 
|
 
|
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|
 
|
 
15%
 
15%
|
 
 
|
 
|
 
|10mM MOPS
 
|10mM MOPS
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|3mM
 
|3mM
 
|10% w/v
 
|10% w/v
|
 
 
|
 
|
 
|
 
|
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|10%
 
|10%
 
|2x 3.5%
 
|2x 3.5%
|5%
+
|5% Sucrose
|
 
 
|pH 6.4->6.2
 
|pH 6.4->6.2
 
|Jessee patent
 
|Jessee patent
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|8%
 
|8%
 
|8%
 
|8%
|
 
 
|
 
|
 
|pH 6.4->6.2
 
|pH 6.4->6.2
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|10%
 
|10%
 
|7%
 
|7%
|
 
 
|10mM MgCl<sub>2</sub>
 
|10mM MgCl<sub>2</sub>
 
|pH 6.4
 
|pH 6.4
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|
 
|
 
|7%
 
|7%
|
 
 
|10mM PIPES pH 6.7
 
|10mM PIPES pH 6.7
 
|pH 5.7->6.2
 
|pH 5.7->6.2
 
|Inoue90
 
|Inoue90
 
|}
 
|}

Latest revision as of 18:00, 2 May 2006

Buffer KCl KOAc CaCl2 MnCl2 RbCl HACoCl3 Glycerol DMSO Other pH Reference
SF 100mM 10mM 50mM 10% pH 6.1 Hanahan patent
RFT 30mM 10mM 50mM 100mM 15% pH 5.8 Hanahan patent
TFB-I 30mM 20mM 50mM 100mM 15% pH 5.8
TFB-II 75mM 10mM

15%

10mM MOPS pH 6.5
FSB 100mM 10mM 45mM 3mM 10% w/v pH 6.2 Hanahan patent
FSB 100mM 10mM 10mM 44mM ? 3mM 10% 2x 3.5% 5% Sucrose pH 6.4->6.2 Jessee patent
FSB 10mM 10mM 45mM 3mM 8% 8% pH 6.4->6.2 Stratagene '525
CCMB80 10mM 80mM 20mM 10% 7% 10mM MgCl2 pH 6.4 Invitrogen '464
TB 250mM 15mM 55mM 7% 10mM PIPES pH 6.7 pH 5.7->6.2 Inoue90