Difference between revisions of "ChIP/Liver harvest"

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{|style="width: 48em; background: #FF6677;"
| align="center"|'''This page is a template and should not be edited.'''<br><span style="font-size:90%">Click [http://openwetware.org/index.php?title={{PAGENAME}}&action=edit here], copy the source, and paste it into your page.</span>
'''Interested in posting a protocol on OpenWetWare?  Here is a template to help you do so.''' 
'''Click the view source tab and copy everything below this line.  Paste it into your new protocol page.  Then replace the text in this page with your own protocol.  Feel free to add or delete sections as appropriate.'''
Replace this sentence with a brief description of the protocol and its goal.
This is a protocol for harvesting mouse liver tissue for [[ChIP|chromatin immunoprecipitation]].  
List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.
*supply 1 (i.e. tubes of a certain size? spreaders?)
*reagent 1
*X &mu;L reagent 2
**component A (reagent 2 is made up of multiple components)
**component B
*equipment 1
*equipment 2
#Step 1
#Step 2
#*Step 2 has some additional information that goes with it.  i.e. Keep at 4&deg;C.
#Step 3
##Step 3 has multiple sub-steps within it.
##Enumerate each of those.
#List troubleshooting tips here. 
* ''Please contribute user notes!''
#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
#Anecdotal observations that might be of use to others can also be posted here. 
Please sign your name to your note by adding <font face="courier"><nowiki>('''~~~~''')</nowiki></font> to the end of your tip.
'''Relevant papers and books'''
* (Young Lab)
<!-- If this protocol has papers or books associated with it, list those references here.  See the [[OpenWetWare:Biblio]] page for more information. -->
#Goldbeter-PNAS-1981 pmid=6947258
#Jacob-JMB-1961 pmid=13718526
#Ptashne-Genetic-Switch isbn=0879697164
*Who has experience with this protocol?
* [[User:Cconboy|cmc]]

Revision as of 10:46, 22 August 2006


This is a protocol for harvesting mouse liver tissue for chromatin immunoprecipitation.




  • Please contribute user notes!


  • (Young Lab)