Cfrench:primerlist: Difference between revisions
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*Restriction sites: SpeI only. | *Restriction sites: SpeI only. | ||
*Notes: replaces TGA stop codon with TAA TAA. | *Notes: replaces TGA stop codon with TAA TAA. | ||
'''Ms_COMTf1''' aat gaattc gcggccgc t tctag atg ggt tca aca ggt gaa ac | |||
*Purpose: forward primer for caffeate O-methltransferase coding sequence from ''Medicago sativa'' (alfalfa). | |||
*Restriction sites: EcoRI, XbaI. | |||
*Notes: eukaryotic gene, no ribosome binding site. | |||
'''Ms_COMTr1''' ct actagt a tta tta aac ctt ctt aag aaa ctc c | |||
*Purpose: reverse primer for caffeate O-methltransferase coding sequence from ''Medicago sativa'' (alfalfa). | |||
*Restriction sites: SpeI only (NOT PstI). | |||
*Notes: adds TAA TAA stop codons. | |||
===Primers for iGEM2007 bioswitches project=== | ===Primers for iGEM2007 bioswitches project=== | ||
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*Purpose: sequencing or amplification of biobrick inserts in pSB1A3 and derivatives thereof. | *Purpose: sequencing or amplification of biobrick inserts in pSB1A3 and derivatives thereof. | ||
*Restriction sites: none (but amplification products will have full biobrick prefix) | *Restriction sites: none (but amplification products will have full biobrick prefix) | ||
'''pSB1A3r1''' agggtggtgacaccttgc | '''pSB1A3r1''' agggtggtgacaccttgc | ||
*Purpose: sequencing or amplification of biobrick inserts in pSB1A3 and derivatives thereof. | *Purpose: sequencing or amplification of biobrick inserts in pSB1A3 and derivatives thereof. | ||
*Restriction sites: none (but amplification products will have full biobrick suffix) | *Restriction sites: none (but amplification products will have full biobrick suffix) | ||
'''pSB1A2insf1''' cgctaaggatgatttctgg | '''pSB1A2insf1''' cgctaaggatgatttctgg | ||
*Purpose: sequencing or amplification of biobrick inserts in pSB1A2 and derivatives thereof. | *Purpose: sequencing or amplification of biobrick inserts in pSB1A2 and derivatives thereof. | ||
*Restriction sites: none (but amplification products will have full biobrick prefix) | *Restriction sites: none (but amplification products will have full biobrick prefix) | ||
*Notes: excellent resuts for sequencing. | |||
'''pSB1A2insr1''' gtcagtgagcgaggaagc | '''pSB1A2insr1''' gtcagtgagcgaggaagc | ||
*Purpose: sequencing or amplification of biobrick inserts in pSB1A2 and derivatives thereof. | *Purpose: sequencing or amplification of biobrick inserts in pSB1A2 and derivatives thereof. | ||
*Restriction sites: none (but amplification products will have full biobrick suffix) | *Restriction sites: none (but amplification products will have full biobrick suffix) | ||
*Notes: excellent resuts for sequencing. | |||
'''bbinsertf1''' attc gcggccgc t tctag | '''bbinsertf1''' attc gcggccgc t tctag | ||
*Purpose: sequencing or amplification of any biobrick insert | *Purpose: sequencing or amplification of any biobrick insert | ||
*Restriction sites: only NotI, but amplification product will have XbaI, and SacI if present. | *Restriction sites: only NotI, but amplification product will have XbaI, and SacI if present. | ||
*Note: gives good results for sequencing, but pSB1A2insf1 is better. | *Note: gives good results for sequencing, but pSB1A2insf1 is better. | ||
'''bbinsertr1''' tgcag cggccgc t actag | '''bbinsertr1''' tgcag cggccgc t actag | ||
*Purpose: sequencing or amplification of any biobrick insert | *Purpose: sequencing or amplification of any biobrick insert | ||
*Restriction sites: only NotI, but amplification product will have SpeI. | *Restriction sites: only NotI, but amplification product will have SpeI. | ||
*Note: usually not very good results for sequencing. | *Note: usually not very good results for sequencing. | ||
'''bbvectorf1''' ag t actag ta gcggccg ctgc | '''bbvectorf1''' ag t actag ta gcggccg ctgc | ||
*Purpose: amplification of vector + biobrick for PCR-based cloning procedures; binds to biobrick suffix and amplifies vector region. | *Purpose: amplification of vector + biobrick for PCR-based cloning procedures; binds to biobrick suffix and amplifies vector region. | ||
*Restriction sites: SpeI, NotI; amplification product will also have PstI. | *Restriction sites: SpeI, NotI; amplification product will also have PstI. | ||
'''bbvectr1''' cctt gagctc taga a gcggccgc gaattc | '''bbvectr1''' cctt gagctc taga a gcggccgc gaattc | ||
*Purpose: amplification of vector + biobrick for PCR-based cloning procedures; binds to biobrick prefix and amplifies vector region. | *Purpose: amplification of vector + biobrick for PCR-based cloning procedures; binds to biobrick prefix and amplifies vector region. | ||
Revision as of 03:29, 27 July 2007
Catalog of primers
Primers for iGEM2007 flavours/fragrances project
echf1 aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c
- Purpose: forward primer for ech (ferA) coding sequence from Ps. fluorescens NCIMB 9046.
- Restriction sites: EcoRI, XbaI
- Notes: no ribosome binding site included.
echr1 ct actagt a tta tta gcg ttt ata ggc ttg cag c
- Purpose: reverse primer for ech (ferA) coding sequence from Ps. fluorescens NCIMB 9046.
- Restriction sites: SpeI only.
- Notes: replaces TGA stop codon with TAA TAA. Also replaces PstI site in coding sequence with silent mutation.
fcsf1 aat gaattc gcggccgc t tctag atg cgc tcc ctg gaa ccc
- Purpose: forward primer for fcs (ferB) coding sequence from Ps. fluorescens NCIMB 9046.
- Restriction sites: EcoRI, XbaI
- Notes: no ribosome binding site included.
fcsr1 ct actagt a tta tta cgg ttt ggg ccc ggc ac
- Purpose: reverse primer for fcs (ferB) coding sequence from Ps. fluorescens NCIMB 9046.
- Restriction sites: SpeI only.
- Notes: replaces TGA stop codon with TAA TAA.
Ms_COMTf1 aat gaattc gcggccgc t tctag atg ggt tca aca ggt gaa ac
- Purpose: forward primer for caffeate O-methltransferase coding sequence from Medicago sativa (alfalfa).
- Restriction sites: EcoRI, XbaI.
- Notes: eukaryotic gene, no ribosome binding site.
Ms_COMTr1 ct actagt a tta tta aac ctt ctt aag aaa ctc c
- Purpose: reverse primer for caffeate O-methltransferase coding sequence from Medicago sativa (alfalfa).
- Restriction sites: SpeI only (NOT PstI).
- Notes: adds TAA TAA stop codons.
Primers for iGEM2007 bioswitches project
revPlacf1 tcta gagctc tgtgtgaaattgttatccg
- Purpose: forward biobrick primer for making a reverse lac promoter.
- Restriction sites: XbaI (probably won't cut as right at end), SacI
revPlacr1 ct actagt a caatacgcaaaccgcctc
- Purpose: reverse biobrick primer for making a reverse lac promoter.
- Restriction sites: SpeI only.
Primers for working with biobricks
pSB1A3f1 tccttagctttcgctaagg
- Purpose: sequencing or amplification of biobrick inserts in pSB1A3 and derivatives thereof.
- Restriction sites: none (but amplification products will have full biobrick prefix)
pSB1A3r1 agggtggtgacaccttgc
- Purpose: sequencing or amplification of biobrick inserts in pSB1A3 and derivatives thereof.
- Restriction sites: none (but amplification products will have full biobrick suffix)
pSB1A2insf1 cgctaaggatgatttctgg
- Purpose: sequencing or amplification of biobrick inserts in pSB1A2 and derivatives thereof.
- Restriction sites: none (but amplification products will have full biobrick prefix)
- Notes: excellent resuts for sequencing.
pSB1A2insr1 gtcagtgagcgaggaagc
- Purpose: sequencing or amplification of biobrick inserts in pSB1A2 and derivatives thereof.
- Restriction sites: none (but amplification products will have full biobrick suffix)
- Notes: excellent resuts for sequencing.
bbinsertf1 attc gcggccgc t tctag
- Purpose: sequencing or amplification of any biobrick insert
- Restriction sites: only NotI, but amplification product will have XbaI, and SacI if present.
- Note: gives good results for sequencing, but pSB1A2insf1 is better.
bbinsertr1 tgcag cggccgc t actag
- Purpose: sequencing or amplification of any biobrick insert
- Restriction sites: only NotI, but amplification product will have SpeI.
- Note: usually not very good results for sequencing.
bbvectorf1 ag t actag ta gcggccg ctgc
- Purpose: amplification of vector + biobrick for PCR-based cloning procedures; binds to biobrick suffix and amplifies vector region.
- Restriction sites: SpeI, NotI; amplification product will also have PstI.
bbvectr1 cctt gagctc taga a gcggccgc gaattc
- Purpose: amplification of vector + biobrick for PCR-based cloning procedures; binds to biobrick prefix and amplifies vector region.
- Restriction sites: SacI, XbaI, EcoRI.
