# Difference between revisions of "Cell cycle analysis"

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[[Image:Theoretical_age_distr.jpg|left|250px]] | [[Image:Theoretical_age_distr.jpg|left|250px]] | ||

From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had notinitiated replication at the time of drug action (4-origin-cells, streaked) and cells that initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from thetheoretical age distribution described by this formula, | From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had notinitiated replication at the time of drug action (4-origin-cells, streaked) and cells that initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from thetheoretical age distribution described by this formula, | ||

− | + | ||

+ | '''F = 2 - 2<sup>((τ-ai)/τ)</sup>''' | ||

+ | |||

where F is the fraction of cells that had not initiated and is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion). | where F is the fraction of cells that had not initiated and is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion). | ||

The C+D period is estimated from the initiation age (ai), the generation time () and the number of generations spanned per cell cycle. | The C+D period is estimated from the initiation age (ai), the generation time () and the number of generations spanned per cell cycle. |

## Revision as of 05:43, 15 August 2008

**Cell cycle analysis of Escherichia coli cells**

C period = the time for a round of chromosome replication D period = the time between the end of a round of chromosome replication and cell division

- Determination of initiation age (a
_{i}) and C+D:

From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had notinitiated replication at the time of drug action (4-origin-cells, streaked) and cells that initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from thetheoretical age distribution described by this formula,

**F = 2 - 2 ^{((τ-ai)/τ)}**

where F is the fraction of cells that had not initiated and is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion).

The C+D period is estimated from the initiation age (ai), the generation time () and the number of generations spanned per cell cycle.