Difference between revisions of "Cell and tissue lysis hub"

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== Related hub pages ==
== Related hub pages ==

Revision as of 03:08, 26 June 2008

protocol hub

This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.

Comparison of lysis methods


  • most efficient method of cell fractionation
  • problem: heat build up which can denature proteins (proportionate to length of sonication)
  • precaution: do on ice and sonicate intermittantly


  • best for animal tissue; less suitable for cells
  • precaution: do on ice to reduce heat build-up and denaturation


  • least effective method
  • plus: does not denature proteins as much as other methods


  • chemical method of lysis
  • problems: detergent may inhibit subsequent reactions
  • problems: detergent may disrupts protein interactions

Specific protocols

description/link target lysis method type of material
Silver: Lysate for Western protein detergent (Triton) eukaryotic cells
Sauer:Lysing E. coli with Lysozymes lysozyme bacteria
Blackburn:Yeast Colony PCR DNA NaOH yeast
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer protein detergent (RIPA buffer) cell line
Streptomyces:Protocols/Mini-Maxi Prep DNA NaOH bacteria
Western Blot/Tissue Preparation protein detergent & physical disruption tissue
Eccles:Protein Lysates from Cells in Culture protein detergent (SDS, NP40) cell line
Eccles:Protein Lysates from Tissue protein detergent, physical, freezing tissue
RNA extraction using trizol/tri RNA phenol cell line
add another method's name target lysis method type of starting material

Related hub pages

Related discussions on BioForum