Difference between revisions of "CTR:Recipes"

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(TE Buffer)
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<div align="right">[http://openwetware.org/wiki/CTR Home]</div>
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<div align="right">[http://openwetware.org/wiki/CTR:Protocols Lab Protocols]</div>
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Lab recipes for general-use buffers. Remember to autoclave! Also refer to the index card box located near the gel rigs.
 
Lab recipes for general-use buffers. Remember to autoclave! Also refer to the index card box located near the gel rigs.
  
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*10.0g N-lauroylsarcosine
 
*10.0g N-lauroylsarcosine
 
*dH<sub>2</sub>O to 1L total volume
 
*dH<sub>2</sub>O to 1L total volume
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<div align="right">[http://openwetware.org/wiki/CTR:Recipes Top]</div>
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<div align="right">[http://openwetware.org/wiki/CTR Home]</div>
  
 
==TAE Buffer (50x)==
 
==TAE Buffer (50x)==
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*57.1mL glacial acetic acid
 
*57.1mL glacial acetic acid
 
*dH<sub>2</sub>O to 1L total volume
 
*dH<sub>2</sub>O to 1L total volume
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<div align="right">[http://openwetware.org/wiki/CTR:Recipes Top]</div>
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<div align="right">[http://openwetware.org/wiki/CTR Home]</div>
  
 
==TBE Buffer (5x)==
 
==TBE Buffer (5x)==
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*3.72g EDTA (or 20mL 0.5M EDTA pH 8.0)
 
*3.72g EDTA (or 20mL 0.5M EDTA pH 8.0)
 
*dH<sub>2</sub>O to 1L total volume
 
*dH<sub>2</sub>O to 1L total volume
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<div align="right">[http://openwetware.org/wiki/CTR:Recipes Top]</div>
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<div align="right">[http://openwetware.org/wiki/CTR Home]</div>
  
 
==TE Buffer==
 
==TE Buffer==
 
*10mM TrisHCl of desired pH
 
*10mM TrisHCl of desired pH
 
*1mM EDTA pH 8.0
 
*1mM EDTA pH 8.0
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<div align="right">[http://openwetware.org/wiki/CTR:Recipes Top]</div>
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<div align="right">[http://openwetware.org/wiki/CTR Home]</div>
  
 
==LowTE Buffer==
 
==LowTE Buffer==
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*3μL EDTA 0.5M
 
*3μL EDTA 0.5M
 
*14.85mL dH<sub>2</sub>O
 
*14.85mL dH<sub>2</sub>O
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<div align="right">[http://openwetware.org/wiki/CTR:Recipes Top]</div>
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<div align="right">[http://openwetware.org/wiki/CTR Home]</div>
  
 
==LB Agar Plates==
 
==LB Agar Plates==
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6. Let plates harden.<br>
 
6. Let plates harden.<br>
 
7. Store in cold room.
 
7. Store in cold room.
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<div align="right">[http://openwetware.org/wiki/CTR:Recipes Top]</div>
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<div align="right">[http://openwetware.org/wiki/CTR Home]</div>

Revision as of 15:42, 28 July 2014

Lab recipes for general-use buffers. Remember to autoclave! Also refer to the index card box located near the gel rigs.

Queen's Lysis Buffer

  • 1.21g Tris Base
  • 0.58g NaCl
  • 3.73g EDTA
  • 10.0g N-lauroylsarcosine
  • dH2O to 1L total volume

TAE Buffer (50x)

  • 242g Tris Base
  • 23.8g EDTA (or 100mL 0.5M EDTA pH 8.0)
  • 57.1mL glacial acetic acid
  • dH2O to 1L total volume

TBE Buffer (5x)

  • 54g Tris Base
  • 27.5g Boric acid
  • 3.72g EDTA (or 20mL 0.5M EDTA pH 8.0)
  • dH2O to 1L total volume

TE Buffer

  • 10mM TrisHCl of desired pH
  • 1mM EDTA pH 8.0

LowTE Buffer

  • 10mM TrisHCl
  • 0.1mM EDTA pH 8.0

example to make 15mL:

  • 150μL TrisHCl 1M
  • 3μL EDTA 0.5M
  • 14.85mL dH2O

LB Agar Plates

1. Mix:

  • 10g Tryptone
  • 5g Yeast extract
  • 10g NaCl
  • 950mL dH2O
  • 15 g/L agar

2. Adjust pH to 7.0 using NaOH and bring volume to 1 L.
3. Autoclave on liquid cycle for 20 minutes at 15 psi.
4. Allow solution to cool to 55°C and add 50 μg/mL of ampicillin or kanamycin.
5. Pour into plates, sterilizing the plate and flask/bottle before each pour.
6. Let plates harden.
7. Store in cold room.