Difference between revisions of "CTR:Notebook/PIRE"

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==Project Description/Abstract==
 
==Project Description/Abstract==
* Place short description of project or notes regarding this project
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* Online lab notebook for PIRE work
  
* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.
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==Files==
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*[http://openwetware.org/images/2/22/BestRAD_Library_Prep_Protocol_April2016.docx BestRAD Library Prep Protocol 96 samples]
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*[http://openwetware.org/wiki/Image:BestRAD_Barcode_Template.xls BestRAD Barcode Sequences]
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*[http://openwetware.org/wiki/Image:BestRad_Adaptor_Information.docx BestRAD Adaptor Information]
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*[http://openwetware.org/images/e/e8/Protocol_for_Annealing_Oligos_BestRad.pdf Protocol for Annealing BestRAD adaptor oligos]
  
==Notes==
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*[http://openwetware.org/images/4/4e/RAD_Illumina_Library_Prep_Protocol_2016-01-26-_For_48_Samples.docx RAD Library Prep Protocol Edited for 48 samples] (updated 2016-01-27)
* Place some notes here for visitors
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*[http://openwetware.org/images/6/62/RAD_Illumina_Library_Prep_Protocol_2015-04-02_2.docx RAD Library Prep Protocol] (updated 2015-04-02)
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**note: NEBNext kit now comes with HiFi Master Mix. High Fidelity 2x Master Mix can still be purchased separately. Both mixes included in kit until June 6, 2015.
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*[https://sites.google.com/site/millermichaelryan Mike Miller's files] (see Downloads section for RAD information kit)
  
* Example: This project is currently on hold until further notice.
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==Notebook==
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* Protocols
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** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2014/04/24 Troubleshooting tests with adapter ligation]
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** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2014/11/21 RAD P1 Adapter Preparation]
  
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* Birds
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** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2014/05/05 RAD Library Prep - Olive Sunbirds Plate 1]
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** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2014/07/09 RAD Library Prep - Olive Sunbirds Plate 2 and Little Greenbuls Plates 1 & 2]
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** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/02/02 RAD Library Prep - Birds Plate 3]
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* Skinks
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** Quantification
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/08/07 Quantification of Skink Extracts for Plate 3]
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** RAD Library Preps
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/01/14 RAD Library Prep - Skinks Plate 1]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/04/01 RAD Library Prep - Skinks Plate 2]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/08/25 RAD Library Prep - Skinks Plate 3 (reruns, 48 well)]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/08/31 RAD Library Prep - Skinks Plate 4 (reruns, 48 samples, with Mice, Frogs, Butterflies)]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/09/09 RAD Library Prep - Skinks Plate 4 Re-prep (reruns, 48 samples, with Butterflies, Frogs, Skinks P5)]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/09/09 RAD Library Prep - Skinks Plate 5 (reruns, 48 samples, with Butterflies, Frogs, Skinks P4)]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/09/15 RAD Library Prep - Skinks Plate 5 Re-prep (reruns, 48 samples)]
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* Mice
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** Quantification
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/06/15 Gel for Praomys Gel-extracted Samples]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/07/30 E-gel for Original Praomys Samples]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/12/13 E-gel for New Praomys Samples sent November 2015]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2016/05/04 E-gel for New Praomys Samples sent May 2016]
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** RAD Library Preps
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/08/31 RAD Library Prep - Mouse Plate 1 (48 samples, with Frogs, Butterflies, Skinks P4)]
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* Frogs
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** Quantification
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2014/11/17 Frog and Mouse DNA QC]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/07/15 E-gel of Plant and Frog Samples]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/07/29 E-gel of Frog Samples]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/12/14 E-gel of New Frog Samples Sent November 2015]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2016/05/05 E-gel of New Frog Samples Sent May 2016]
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** RAD Library Preps
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/08/31 RAD Library Prep - Frogs Plate 1 (48 samples, with Butterflies, Mice, Skinks P4)]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/09/09 RAD Library Prep - Frogs Plate 1 Re-prep (48 samples, with Butterflies, Skinks P4, Skinks P5)]
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* Butterflies
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** Quantification
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/07/17 E-gel_of_B.dorothea_Extractions]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2016/05/19 E-gel_of_B.dorothea_Extractions sent in May 2016]
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** RAD Library Preps
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/08/31 RAD Library Prep - Butterflies Plate 1 (48 samples, with Frogs, Mice, Skinks P4)]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/09/09 RAD Library Prep - Butterflies Plate 1 Re-prep (48 samples, with Frogs, Skinks P4, Skinks P5)]
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2016/02/16 RAD Library Prep - Butterflies Plate 2 (96 samples, Original RAD protocol)]
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** BESTRAD Library Preps
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2016/02/21 Butterfly Plate 2 (96 samples, BestRAD protocol)]
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*Plants
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** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2015/09/23 Assessment of Plant DNA quality sent from Alexandra Ley 04-2015]
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*Duiker
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** Quantification
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2016/06/29 Assessment of Duiker DNA quality sent from UNO 05-2016]
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** BESTRAD Library Prep
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*** [http://openwetware.org/wiki/CTR:Notebook/PIRE/2016/08/29 Plate 1 (38 samples, BestRAD protocol)]
  
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Latest revision as of 20:03, 23 September 2017

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Project Description/Abstract

  • Online lab notebook for PIRE work

Files

Notebook