CH391L/S2013

Molecular Mechanisms of Genome Maintenance

CH 391L (Spring 2013)

Unique # 52955

Wednesdays 2–5 PM, WEL 3.422, Professor: Finkelstein

Instructor: Dr. Ilya Finkelstein

Office Hours: by appointment only

Office: MBB 3.422AA

Syllabus

Index of All CH391/S2013 Pages

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Introduction

Topics: This course will focus on current developments in understanding the molecular mechanisms of genome maintenance and cancerogenesis. A strong emphasis of the course will be to understand the methods and techniques that have enabled modern breakthroughs in understanding genome stability. The course will rely heavily on primary literature and class presentations.

Prerequisite: Biochemistry (CH339K and CH339L) or equivalent. Undergraduate students must have the instructor's permission to register for this course.

Course web page: The course web site on the OpenWetWare (OWW) Wiki will be used to complete assignments. Participants will be required to register an account on OWW and learn to edit this Wiki.

Assignments

A Wiki Editing Tutorial borrowed from (The Other) CH391L

Due January 23, 2013

1. Alesha S: Spontaneous DNA breakage in single living Escherichia coli cells.
   1. First oral presentation
   2. Wiki article -- first draft
2. Tanya R: Activation of DSB Processing Requires Phosphorylation of CtIP by ATR.
   1. First oral presentation
   2. Wiki article -- first draft
      1. CH391L/S2013 Tanya Raymond Jan 23 2013
3. Hala Ouzon: Purified human BRCA2 stimulates RAD51-mediated recombination.
   1. First oral presentation
   2. Wiki article -- first draft
      1. CH391L/S2013 Hala Ouzon Jan 23 2013

Due January 30, 2013

1. Alesha S: Spontaneous DNA breakage in single living Escherichia coli cells.
   1. Second oral presentation
   2. Wiki article -- final draft
2. Tanya R: Activation of DSB Processing Requires Phosphorylation of CtIP by ATR.
   1. Second oral presentation
   2. Wiki article -- final draft
3. Hala Ouzon: Purified human BRCA2 stimulates RAD51-mediated recombination.
   1. Second oral presentation
   2. Wiki article -- final draft
4. Logan M: Activation of the cellular DNA damage Response in the Absence of DNA Lesions.
   1. First oral presentation
   2. Wiki article -- first draft
      1. CH391L/S2013 Logan R Myler Jan 30 2013

Due February 13, 2013

Class Assignments

CH391L/S2013/SuggestedTopics

Suggested Reading

A Brief Overview of Cancer Biology

1. The hallmarks of cancer. Hanahan D, Weinberg RA. Cell. 2000 Jan 7;100(1):57-70.
2. Hallmarks of cancer: the next generation. Hanahan D, Weinberg RA. Cell. 2011 Mar 4;144(5):646-74.

DNA Double Strand Break (DSB) Repair

1. Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiae. Niu H, Chung WH, Zhu Z, Kwon Y, Zhao W, Chi P, Prakash R, Seong C, Liu D, Lu L, Ira G, Sung P. Nature. 2010 Sep 2;467(7311):108-11.
2. Spontaneous DNA breakage in single living Escherichia coli cells. Pennington JM, Rosenberg SM. Nat Genet. 2007 Jun;39(6):797-802. Epub 2007 May 27.
3. Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cells. Pierce AJ, Hu P, Han M, Ellis N, Jasin M. Genes Dev. 2001 Dec 15;15(24):3237-42.
4. Rad51 paralogues Rad55-Rad57 balance the antirecombinase Srs2 in Rad51 filament formation. Liu J, Renault L, Veaute X, Fabre F, Stahlberg H, Heyer WD. Nature. 2011 Oct 23;479(7372):245-8.
5. Purified human BRCA2 stimulates RAD51-mediated recombination. Jensen RB, Carreira A, Kowalczykowski SC. Nature. 2010 Oct 7;467(7316):678-83.
6. Direct imaging of RecA nucleation and growth on single molecules of SSB-coated ssDNA. Bell JC, Plank JL, Dombrowski CC, Kowalczykowski SC. Nature. 2012 Nov 8;491(7423):274-8.
7. Identification of a DNA nonhomologous end-joining complex in bacteria. Weller GR, Kysela B, Roy R, Tonkin LM, Scanlan E, Della M, Devine SK, Day JP, Wilkinson A, d'Adda di Fagagna F, Devine KM, Bowater RP, Jeggo PA, Jackson SP, Doherty AJ. Science. 2002 Sep 6;297(5587):1686-9.

Class Participants

User:Ilya J. Finkelstein

User:Alesha Stewart

User:Tanya_E_Raymond

User:Hala Ouzon-Shubeita

User:Logan_R._Myler