Difference between revisions of "CH391L/S13/In vitro Selection of FNAs"

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===Deoxyribozymes===
 
===Deoxyribozymes===
 
[[Image:8-17OP.png]]
 
[[Image:8-17OP.png]]
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===Aptamers and Riboswitches===
  
  

Revision as of 02:26, 11 February 2013


Introduction

Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues) that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism.


1st-Ribozymes-discovered2.png

Functional Nucleic Acids

[1],[2]

Ribozymes

Deoxyribozymes

8-17OP.png


Aptamers and Riboswitches

In vitro Selection of Functional Nucleic Acids

In-vitro-selection.png





Extra

Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.

CH391L S12 Phosphoramidite.png

Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length. <biblio>

  1. Cech1982 pmid=6297745
  2. Altman1983 pmid=6197186

<\biblio>