Difference between revisions of "CH391L/S13/In vitro Selection of FNAs"

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==Introduction==
 
==Introduction==
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Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues)  that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism.  
 
Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues)  that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism.  
  
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[[Image:1st-Ribozymes-discovered2.png|600px|]]
  
 
==Functional Nucleic Acids==
 
==Functional Nucleic Acids==
  
<cite>Cech1982</cite>
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<cite>Cech1982</cite>,<cite>Altman1982</cite>
 
==In vitro Selection of Functional Nucleic Acids==
 
==In vitro Selection of Functional Nucleic Acids==
  
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Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.
 
Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.
 +
<biblio>
  
 
#Cech1982 pmid=6297745
 
#Cech1982 pmid=6297745
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#Altman1983 pmid=6197186
 +
 +
<\biblio>

Revision as of 23:41, 10 February 2013


Introduction

Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues) that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism.


1st-Ribozymes-discovered2.png

Functional Nucleic Acids

[1],[2]

In vitro Selection of Functional Nucleic Acids

Ribozymes

Deoxyribozymes

Extra

Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.

CH391L S12 Phosphoramidite.png

Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length. <biblio>

  1. Cech1982 pmid=6297745
  2. Altman1983 pmid=6197186

<\biblio>