CH391L/S13/In vitro Selection of FNAs

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Introduction

Types of Functional Nucleic Acids

In vitro Selection of Functional Nucleic Acids

Ribozymes

Deoxyribozymes

Aptamers

Extra

Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.

CH391L S12 Phosphoramidite.png

Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.