CH391L/S12/In vitro Selection
- 1 Overview of in vitro selection
- 1.1 Library Generation
- 1.2 Increased Representation
- 1.3 Confinement of Function
- 2 ===In vitro Compartmentalization=======History
- 3 References
Overview of in vitro selection
Once diversity is created, the selection must must allow function variants to become a larger percentage of the pool. This step is often the most difficult to design.
The easiest function to enrich for is affinity for a ligand. To select for binders (aptamers for nucleic acids; antibodies and others for proteins,) one exposes the pool of potential binders to a fixed ligand. The best binders affix to the ligand while weaker binders are washed away. Those that remain can be amplified for further round of selection. For nucleic acids, the binder itself can be subject to amplification, as it is both the information-carrying and function-carrying molecule. For protein binders, the scheme must include linking of the information-carrying nucleic acid to the function-carrying protein. Some examples of this linking are phage display, cell-surface display, and ribosome display.
Confinement of Function
===In vitro Compartmentalization=======History
DNA Modifying Proteins
Other Enzymatic Functions