CH391L/S12/In vitro Selection: Difference between revisions
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===Library Generation=== | ===Library Generation=== | ||
=====Randomized Oligodeoxynucleotides===== | |||
=====Mutagenic PCR===== | |||
=====Gene Shuffling===== | |||
=====Neutral Drift===== | |||
===Confinement of Function=== | ===Confinement of Function=== | ||
=====Binding===== | |||
=====Cis-action===== | |||
=====Cellularization===== | |||
=====''In vitro'' Compartmentalization===== | |||
===Increased Representation=== | ===Increased Representation=== | ||
=====Binding===== | |||
=====Protection===== | |||
=====Selective Amplification===== | |||
=====FACS===== | |||
==History== | ==History== | ||
===Aptamers=== | ===Nucleic Acids=== | ||
===Ribozymes=== | =====Aptamers===== | ||
===DNA Modifying Proteins=== | Ellington 1990 | ||
===Other Enzymatic Functions=== | =====Ribozymes===== | ||
Bartel 1993 | |||
===Proteins=== | |||
=====Antibodies===== | |||
Accepting suggestions | |||
=====DNA Modifying Proteins===== | |||
Tawfik 1998 | |||
=====Other Enzymatic Functions===== | |||
Griffiths 2003 | |||
===Self-Replication=== | ===Self-Replication=== | ||
Ghadessy 2001 | |||
==References== | ==References== | ||
Revision as of 21:45, 27 January 2012
Overview of in vitro selection
Library Generation
Randomized Oligodeoxynucleotides
Mutagenic PCR
Gene Shuffling
Neutral Drift
Confinement of Function
Binding
Cis-action
Cellularization
In vitro Compartmentalization
Increased Representation
Binding
Protection
Selective Amplification
FACS
History
Nucleic Acids
Aptamers
Ellington 1990
Ribozymes
Bartel 1993
Proteins
Antibodies
Accepting suggestions
DNA Modifying Proteins
Tawfik 1998
Other Enzymatic Functions
Griffiths 2003
Self-Replication
Ghadessy 2001
