Difference between revisions of "CAPS"

From OpenWetWare
Jump to: navigation, search
m (Forgot some things,.)
(had conflicting pH's, fixed)
 
(One intermediate revision by the same user not shown)
Line 9: Line 9:
 
#* 11.066g CAPS
 
#* 11.066g CAPS
 
#* 4.5L 10% Methanol
 
#* 4.5L 10% Methanol
# Adjust pH to 7.4 with around 5mL concentrated HCl
+
# Adjust pH to 11 with around 5mL 10N NaOH
 
# Add 10% Methanol to 5L
 
# Add 10% Methanol to 5L
  
[[Category:Material]] and [[Category:Buffers]]
+
[[Category:Material]] [[Category:Buffers]]

Latest revision as of 11:04, 6 May 2009

Purpose

CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol.

Recipe

1X CAPS is 10mM, pH 11

To make 5L of 1X CAPS,

  1. Combine the following:
    • 11.066g CAPS
    • 4.5L 10% Methanol
  2. Adjust pH to 11 with around 5mL 10N NaOH
  3. Add 10% Methanol to 5L