Difference between revisions of "CAPS"

From OpenWetWare
Jump to: navigation, search
m (Forgot some things,.)
m (Recipe: removed superfluous and)
Line 12: Line 12:
# Add 10% Methanol to 5L
# Add 10% Methanol to 5L
[[Category:Material]] and [[Category:Buffers]]
[[Category:Material]] [[Category:Buffers]]

Revision as of 15:49, 21 April 2009


CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol.


1X CAPS is 10mM, pH 11

To make 5L of 1X CAPS,

  1. Combine the following:
    • 11.066g CAPS
    • 4.5L 10% Methanol
  2. Adjust pH to 7.4 with around 5mL concentrated HCl
  3. Add 10% Methanol to 5L