CAPS: Difference between revisions
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# Add 10% Methanol to 5L | # Add 10% Methanol to 5L | ||
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Revision as of 16:49, 21 April 2009
Purpose
CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol.
Recipe
1X CAPS is 10mM, pH 11
To make 5L of 1X CAPS,
- Combine the following:
- 11.066g CAPS
- 4.5L 10% Methanol
- Adjust pH to 7.4 with around 5mL concentrated HCl
- Add 10% Methanol to 5L
