Difference between revisions of "Buckner Lab:Notebook/Summer 2010/2010/05/19"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2010/05/19 Entry for Buckner_Lab:Notebook/Summer_2010)
 
Line 1: Line 1:
 
{|{{table}} width="800"
 
{|{{table}} width="800"
 
|-
 
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">DNA Purification and Gel Electrophoresis</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
+
==Plasmid Isolation==
* Insert content here...
+
* Used QIAprep isolation kit from Qiagen to isolate plasmids.
 +
* Performed EcoR1 digestion to isolated DNA for ~2.25 hrs
 +
 
 +
==Pouring and Running Gel==
 +
* Made a 0.8% agarose gel with ethidium bromide
 +
* Ran the gel for 1.5 hrs in TAE buffer at 60V.
 +
* Photographed and annotated gels
 +
 
 +
-Didn't allow the gel to run long enough. <br>
 +
-Need to add all of the DNA from the prep tube when taking a "look" after an EcoRI digestion
  
  

Revision as of 11:55, 21 May 2010

Owwnotebook icon.pngDNA Purification and Gel Electrophoresis <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Plasmid Isolation

  • Used QIAprep isolation kit from Qiagen to isolate plasmids.
  • Performed EcoR1 digestion to isolated DNA for ~2.25 hrs

Pouring and Running Gel

  • Made a 0.8% agarose gel with ethidium bromide
  • Ran the gel for 1.5 hrs in TAE buffer at 60V.
  • Photographed and annotated gels

-Didn't allow the gel to run long enough.
-Need to add all of the DNA from the prep tube when taking a "look" after an EcoRI digestion