Bryan Hernandez

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Construction and Characterization of Riboregulators

A Riboregulator is a post-transcriptional regulator. Its mechanism for regulation involves the occlusion of the Ribosome via a hairpin on the mRNA transcript in the 'off' state thereby preventing (or 'locking') translation of the ORF. The 'on' state is recovered by introducing a complementary sequence (or 'key') that disrupts the hairpin on the mRNA transcript allowing the Ribosome to bind and initiate translation. To learn more, go here.

Addressable Conjugation in Bacterial Networks

iGEM UC Berkeley

  • Our project was to create an addressable cell-to-cell communication mechanism in e. coli.



The Sortostat is a microfluidic chemostat integrated with a cell sorter. My project consists of demonstrating the sortostat's chemostat functionality, a technique rarely seen in microfluidics.

Immediate Goals

  • Debug all engineering-related problems with sortostat.
  • Demonstrate Sortostat's ability to attain chemostasis in a population of E. Coli.
  • Demonstrate Sortostat's ability to sort two phenotypically different populations of E. Coli. In this case, I will be using a mixture of E. Coli expressing CFP and YFP.
  • Demostrate Sortostat's ability to select for an arbitrary population of cells that are phenotypically different from others and maintain this population at a steady state.
  • UROP Proposal
  • Chemostat Theory
  • Sortostat/Experiments
  • Sortostat/Growth Tests

-80 stocks


  • I am currently an undergraduate researcher in the Endy Lab at MIT. I am currently building and characterizing orthogonal riboregulators. Learn more about riboregulators and the work I have done here
  • MIT Class of 2009; Majoring in Mathematics and Biological Engineering.