Difference between revisions of "Bromophenol blue"

From OpenWetWare
Jump to: navigation, search
m
m
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 +
[[Image:Agarose gel dyes.jpg|right|200px]]
 +
 
'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydroxyl groups).
 
'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydroxyl groups).
  
 
Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.
 
Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.
  
* 500bp at 1% agarose
+
* 1000bp at 0.7% agarose
 +
* 500bp at 1%
 
* 150bp at 2%
 
* 150bp at 2%
 
* 50bp at 3%  
 
* 50bp at 3%  

Latest revision as of 06:18, 3 June 2009

Agarose gel dyes.jpg

Bromophenol blue (BPB) is most commonly used as an agarose gel electrophoresis size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than Xylene cyanol (539g/mol) because of its larger negative charge (hydroxyl groups).

Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.

  • 1000bp at 0.7% agarose
  • 500bp at 1%
  • 150bp at 2%
  • 50bp at 3%

See also

External links