Difference between revisions of "Bromophenol blue"

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'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] because of 1 net negative charge.
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[[Image:Agarose gel dyes.jpg|right|200px]]
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'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydroxyl groups).
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Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.
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* 1000bp at 0.7% agarose
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* 500bp at 1%
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* 150bp at 2%
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* 50bp at 3%
  
 
== See also ==
 
== See also ==
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== External links ==
 
== External links ==
 
* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia]
 
* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia]
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[[Category:Material]] [[Category:Dye]] [[Category:Agarose gel electrophoresis]]

Latest revision as of 06:18, 3 June 2009

Agarose gel dyes.jpg

Bromophenol blue (BPB) is most commonly used as an agarose gel electrophoresis size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than Xylene cyanol (539g/mol) because of its larger negative charge (hydroxyl groups).

Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.

  • 1000bp at 0.7% agarose
  • 500bp at 1%
  • 150bp at 2%
  • 50bp at 3%

See also

External links