Difference between revisions of "Bromodeoxyuridine (BrdU)"

From OpenWetWare
Jump to: navigation, search
m
m
 
Line 4: Line 4:
  
 
== Mechanism ==
 
== Mechanism ==
BrdU is phosphorylated by cells to BrdUTP. This precursor is incorporated into newly synthesised DNA during S phase instead of deoxythymidine triphosphate (dTTP). It can then be detected with an anti-BrdU antibody coupled to a fluorophore or an enzyme. Other detection methods included BrdU quenching of DNA fluorophores and selective photolysis of BrdU-containing DNA.
+
BrdU is phosphorylated by cells to BrdUTP. This precursor is incorporated into newly synthesised DNA during S phase instead of deoxythymidine triphosphate (dTTP). It can then be detected with an anti-BrdU antibody coupled to a fluorophore or an enzyme. Other detection methods include BrdU quenching of DNA fluorophores and selective photolysis of BrdU-containing DNA.
  
 
== Examples ==
 
== Examples ==

Latest revision as of 15:45, 5 January 2010

BrdU next to the deoxynucleoside deoxythymidine (dT) it can replace during DNA synthesis

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside analogue of thymidine. BrdU is commonly used to detect cell proliferation.

Mechanism

BrdU is phosphorylated by cells to BrdUTP. This precursor is incorporated into newly synthesised DNA during S phase instead of deoxythymidine triphosphate (dTTP). It can then be detected with an anti-BrdU antibody coupled to a fluorophore or an enzyme. Other detection methods include BrdU quenching of DNA fluorophores and selective photolysis of BrdU-containing DNA.

Examples

See also