Difference between revisions of "Blackburn:Yeast Colony PCR"

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(Materials)
m (Materials)
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*Standard PCR machine, tubes*Qiagen Taq with Q-solution
 
*Standard PCR machine, tubes*Qiagen Taq with Q-solution
*[http://www1.qiagen.com/Products/Pcr/TaqSystem/TaqDnaPolymerase.aspx Qiagen Taq Polymerase Kit with Q-solution]
+
*[http://www.qiagen.com/Products/Pcr/TaqSystem/TaqDnaPolymerase.aspx Qiagen Taq Polymerase Kit with Q-solution]
 
*A small yeast colony
 
*A small yeast colony
 
*0.02M NaOH (3uL per reaction)
 
*0.02M NaOH (3uL per reaction)

Revision as of 20:01, 11 June 2006

Overview

This is a quick and easy yeast colony PCR protocol that does not require zymolyase step.

Materials

Procedure

  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.

Notes

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding ('''~~~~''') to the end of your tip.

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. PubMed ID:6947258 | HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. PubMed ID:13718526 | HubMed [Jacob-JMB-1961]
  3. Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. ISBN:0879697164 [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed | HubMed

Contact

  • Who has experience with this protocol?