Bitan:Electron microscopy

Electron Microscopy

Glow discharge the grid

1. Clean the jar.
2. Adjust the height of the sample holder.
3. Assemble the circular electrode in the chamber over the holder.
4. Place grids in the center of the holder, close the chamber.
5. Switch the button to HTS.
6. Turn off (tight) the gas release.
7. Turn on the pump unit.
8. Switch the button to glow.
9. Check the glow time is 60s
10. Click Main button.
11. Release the gas carefully to keep the pressure at 60 mba
12. START
13. Turn the charge switch to 4-5
14. 60s later turn the charge back to 0.
15. Release the gas.
16. Turn off the Main button.
17. After the gas is balanced, unclip the bell jar and take out the grids.
18. Put the jar back.
19. Sign on notebook.

Sample loading

1. Take UAc 4% and glutaraldehyde 25% from refrigerate.
2. Dilute UAc to 1% and glutaraldehyde to 2.5%.
3. Clip the edge of discharged grid with tweezer.
4. Apply 8 μL of sample solution onto the grid carefully and incubate for 10 min. (the incubation time is determined by the sample property and concentration.)
5. Remove the solution with Whatman paper.
6. Apply 5 μL of 2.5% glutaraldehyde onto the grid and then incubate for 10 min.
7. Wick off the solution with Whatman paper.
8. Apply 5 μL of 1% glutaraldehyde onto the grid; incubate for 10 min.
9. Remove the solution with Whatman paper and air-dry the grid.
10. Place the grid into a gird-storage box.