Difference between revisions of "Biomolecular Breadboards:Protocols"
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=== Cost estimate === | === Cost estimate === | ||
| − | The cell-free circuit breadboard costs approximately | + | The cell-free circuit breadboard costs approximately 2.64 cents per uL, or 31.7 cents per 12 uL reaction. The cost distribution is shown in the table below. |
| − | {| border="1" cellpadding="5" style="width: | + | {| border="1" cellpadding="5" style="width: 800px" |
! scope="col" | Component | ! scope="col" | Component | ||
| − | ! scope="col" | | + | ! scope="col" | Material price per μL of reaction |
| − | ! scope="col" | | + | ! scope="col" | Labor price per μL of reaction |
| + | ! scope="col" | Total price per μL of reaction | ||
| + | ! scope="col" | Total price per 12 μL reaction | ||
|- | |- | ||
| − | | style="width: 200px;" | ''E. coli'' crude extract || style="text-align:center; width: 100px;" | 0.45 cents || style="text-align:center; width: 100px;" | | + | | style="width: 200px;" | ''E. coli'' crude extract || style="text-align:center; width: 100px;" | 0.45 cents || style="text-align:center; width: 100px;" | 0.62 cents || style="text-align:center; width: 100px;" | 1.07 cents || style="text-align:center; width: 100px;" | 12.84 cents |
|- | |- | ||
| − | | Amino acids || style="text-align:center" | 0.21 cents || | + | | Amino acids || style="text-align:center" | 0.21 cents || style="text-align:center" | 0.034 cents || style="text-align:center" | 0.244 cents || style="text-align:center" | 2.93 cents |
|- | |- | ||
| − | | 3-PGA || style="text-align:center" | 0.39 cents || | + | | 3-PGA || style="text-align:center" | 0.39 cents || style="text-align:center" | 0.94 cents || style="text-align:center" | 1.33 cents || style="text-align:center" | 15.96 cents |
|- | |- | ||
| − | | '''Total price''' || style="text-align:center" | 1.05 cents || style="text-align:center" | | + | | '''Total price''' || style="text-align:center" | 1.05 cents || style="text-align:center" | 1.6 cents || style="text-align:center" | '''2.64 cents''' || '''31.7 cents''' |
|} | |} | ||
For a more detailed cost estimate, please see [[Biomolecular Breadboards:Protocols:cost_estimate|Breadboard Cost Estimate]]. | For a more detailed cost estimate, please see [[Biomolecular Breadboards:Protocols:cost_estimate|Breadboard Cost Estimate]]. | ||
Revision as of 23:48, 12 August 2012
| Home | Protocols | DNA parts | Preliminary Data | Models | More Info |
Cell-free circuit breadboard
The following protocols are available for the cell-free circuit breadboard:
- Reaction Preparation - Cell-free expression of circuits from linear or plasmid DNA
Cost estimate
The cell-free circuit breadboard costs approximately 2.64 cents per uL, or 31.7 cents per 12 uL reaction. The cost distribution is shown in the table below.
| Component | Material price per μL of reaction | Labor price per μL of reaction | Total price per μL of reaction | Total price per 12 μL reaction |
|---|---|---|---|---|
| E. coli crude extract | 0.45 cents | 0.62 cents | 1.07 cents | 12.84 cents |
| Amino acids | 0.21 cents | 0.034 cents | 0.244 cents | 2.93 cents |
| 3-PGA | 0.39 cents | 0.94 cents | 1.33 cents | 15.96 cents |
| Total price | 1.05 cents | 1.6 cents | 2.64 cents | 31.7 cents |
For a more detailed cost estimate, please see Breadboard Cost Estimate.
